Jeg-3 cells lacking ERM proteins or LOK/SLK are viable and lack microvilli.(A) Protein expression using the indicated antibodies in Western blots of ERM proteins and LOK and SLK in Jeg-3 CRISPR cell lysates. HeLa cell lysate was used as a control for expression of moesin, which is not present in Jeg-3. Tubulin expression was used as a loading control. (B) Jeg-3 cell lines lacking ERM expression or LOK and SLK expression were stained with WGA, ezrin, and actin. Yellow arrowheads indicate strong membrane ruffles. (C) Percentage of cells expressing microvilli. Bars represents mean ± SEM; n = 3. P values were calculated with Welch’s t test (*, P ≤ 0.05; ***, P ≤ 0.001). (D) Staining of cells with EBP50, ezrin, and actin in the indicated cells. Scale bars, 10 µm unless otherwise noted. Vertical sections were expanded threefold for clarity.
Figure 1.

Jeg-3 cells lacking ERM proteins or LOK/SLK are viable and lack microvilli. (A) Protein expression using the indicated antibodies in Western blots of ERM proteins and LOK and SLK in Jeg-3 CRISPR cell lysates. HeLa cell lysate was used as a control for expression of moesin, which is not present in Jeg-3. Tubulin expression was used as a loading control. (B) Jeg-3 cell lines lacking ERM expression or LOK and SLK expression were stained with WGA, ezrin, and actin. Yellow arrowheads indicate strong membrane ruffles. (C) Percentage of cells expressing microvilli. Bars represents mean ± SEM; n = 3. P values were calculated with Welch’s t test (*, P ≤ 0.05; ***, P ≤ 0.001). (D) Staining of cells with EBP50, ezrin, and actin in the indicated cells. Scale bars, 10 µm unless otherwise noted. Vertical sections were expanded threefold for clarity.

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