The ESCRT machinery is essential for the degradation of plasma protein Hxt3 during glucose starvation. (A) Western blots showing the degradation of Hxt3-GFP after glucose starvation in the indicated strains. Asterisk indicates a minor cleavage product from full-length protein. (B) Quantification (±SD, n = 3) of protein levels in A. (C–H) Subcellular localization of Hxt3-GFP before (0 h) and after (3 h) glucose starvation in WT (C), vps4Δ (D), vps27Δ (E), vps23Δ (F), vps36Δ (G), and snf7Δ (H) cells. (I and J) Colocalization of Hxt3-GFP with Hse1-mCherry (I) and Vps4-3HA-mCherry (J) during glucose starvation. Dashed lines indicate the periphery of yeast cells. Scale bars, 3 µm.