Figure 7.
PpV is regulated by the Hh signaling. (A–A′′′′) Shown are expression patterns of PpV, CiFL, and Smo in a WT wing disc. Enlarged boxed area is shown in A′–A′′′′. (B and B′) The expression of PpV, CiFL, and Smo shown in B was analyzed with ImageJ. The horizontal axis represents the a-p orientation (anterior to the left and posterior to the right). Dashed line indicates the a-p boundary. Anterior compartment cells responding to low, intermediate, and high levels of Hh signaling are shown in yellow, green, and violet, respectively. Cells responding to the highest Hh signaling are shown in purple. A higher amount of PpV protein was found in anterior cells, with the exception of the posterior-most cells in anterior compartment (highlighted in purple), where PpV protein accumulation declined sharply. (C–C2′′′) Increased amount of PpV protein was found in loss-of-function ptcS2 somatic clones (marked by the absence of GFP) away from (i.e., low-to-intermediate Hh signaling; C1–C1′′′) but not abutting the a-p boundary (i.e., high-level signaling; C2–C2′′′). Enlarged boxed areas (C) are shown (C1–C1′′′ and C2–C2′′′). Both PpV expression (C1′′) and CiFL stabilization (C1′′′) were increased in ptcS2 clones away from the a-p boundary. In clones abutting the boundary, PpV expression was not altered, but the amount of CiFL was reduced, probably due to conversion of CiFL to labile CiA in response to high-level Hh signaling. Dotted lines mark ptcS2 clones (C1, C1′, C2, and C2′). (D) Schematic drawing of a WT wing disc and a wing disc expressing ptc RNAi. At the a-p boundary of a WT wing disc, CiFL was converted to an active but labile form (i.e., lower level) of CiA. Upon overexpressing ptc RNAi by ap-Gal4 in dorsal compartment, the a-p boundary displaying high levels of Hh signaling (i.e., with labile Ci) was expanded. (E–E′′) PpV protein production was reduced in expanded region (yellow arrows) labeled by labile CiFL when ptc RNAi was expressed by ap-Gal4 in a wing disc. (F–F′′′) PpV protein production was restored in ptc RNAi-induced expanded region (yellow arrows) labeled by labile CiFL when en RNAi was expressed by ap-Gal4 in a wing disc. Scale bars, A and C–C′′′, 100 µm; A′–A′′′′, B, C1–C2′′′, and E–F′′′, 25 µm.

PpV is regulated by the Hh signaling. (A–A′′′′) Shown are expression patterns of PpV, CiFL, and Smo in a WT wing disc. Enlarged boxed area is shown in A′–A′′′′. (B and B′) The expression of PpV, CiFL, and Smo shown in B was analyzed with ImageJ. The horizontal axis represents the a-p orientation (anterior to the left and posterior to the right). Dashed line indicates the a-p boundary. Anterior compartment cells responding to low, intermediate, and high levels of Hh signaling are shown in yellow, green, and violet, respectively. Cells responding to the highest Hh signaling are shown in purple. A higher amount of PpV protein was found in anterior cells, with the exception of the posterior-most cells in anterior compartment (highlighted in purple), where PpV protein accumulation declined sharply. (C–C2′′′) Increased amount of PpV protein was found in loss-of-function ptcS2 somatic clones (marked by the absence of GFP) away from (i.e., low-to-intermediate Hh signaling; C1–C1′′′) but not abutting the a-p boundary (i.e., high-level signaling; C2–C2′′′). Enlarged boxed areas (C) are shown (C1–C1′′′ and C2–C2′′′). Both PpV expression (C1′′) and CiFL stabilization (C1′′′) were increased in ptcS2 clones away from the a-p boundary. In clones abutting the boundary, PpV expression was not altered, but the amount of CiFL was reduced, probably due to conversion of CiFL to labile CiA in response to high-level Hh signaling. Dotted lines mark ptcS2 clones (C1, C1′, C2, and C2′). (D) Schematic drawing of a WT wing disc and a wing disc expressing ptc RNAi. At the a-p boundary of a WT wing disc, CiFL was converted to an active but labile form (i.e., lower level) of CiA. Upon overexpressing ptc RNAi by ap-Gal4 in dorsal compartment, the a-p boundary displaying high levels of Hh signaling (i.e., with labile Ci) was expanded. (E–E′′) PpV protein production was reduced in expanded region (yellow arrows) labeled by labile CiFL when ptc RNAi was expressed by ap-Gal4 in a wing disc. (F–F′′′) PpV protein production was restored in ptc RNAi-induced expanded region (yellow arrows) labeled by labile CiFL when en RNAi was expressed by ap-Gal4 in a wing disc. Scale bars, A and C–C′′′, 100 µm; A′–A′′′′, B, C1–C2′′′, and E–F′′′, 25 µm.

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