Hypothetical model for the flux of sterols in fission yeast cells. Sterol molecules are transported in the cell by ways orthogonal to vesicular trafficking. The StART-like domain Ltc1 is necessary for sterol retrograde transport from the PM, likely to the ER. Sterols then move in an anterograde manner that bypasses the Golgi to endosomes. From the endosome, sterols return to the PM independently of vesicular secretion, either through the ER or more directly. Arp2/3-dependent F-actin assembly actively promotes the transport and/or retention of sterols to the PM.