The D4H sterol bio-sensor labels the plasma membrane in fission yeast cells. (A) mCherry-D4H distribution during interphase growth in S. pombe cells. The arrowheads point to an internal dot at 58’ and clearance of D4H from the predivisional site at 106’. Time in minutes. (B) Localization of D4H and D4 sterol biosensors in vegetative cells. The plot on the right shows the PM to cytoplasm fluorescence ratio. Horizontal bars indicate the mean. (C) mCherry-D4H distribution in cells treated with the sterol biosynthesis inhibitor terbinafine for 16 h. (D) Recovery of mCherry-D4H PM localization upon wash-out of terbinafine after 16 h treatment. (E) mCherry-D4H colocalizes with the PM marker GFP-RitC. (F) mCherry-D4H decorates the PM, not the ER marked with GFP-AHDL in WT and scs2Δ scs22Δ mutants deficient in ER-PM attachment. Arrowheads point to zones of PM devoid of ER. (G) Distinct distribution of mCherry-D4H and filipin staining in cells also coexpressing GFP-Tna1. Scale bars, 2 µm.