Analysis of SG Rib-binding data implicates ribosome/translation. (A) Rib-binding signal enrichment between the GAL4 drivers comparing all overlapping peaks (n = 1,597) from fkh-GAL4 > UAS-rib-GFP and sage-GAL4 > UAS-rib-GFP yields a Pearson correlation coefficient of 0.343 (left). A comparison of peaks after implementation of the IDR pipeline on biological replicas yields high positive correlation (Pearson correlation coefficient = 0.6877): high-confidence Rib-binding to SG targets (n = 436; right). (B) Binding signal feature analysis of individual and combined driver experiments reveals that ∼75% of Rib binding peaks localize within the promoters or 5′ UTRs. Controls indicate expected distribution of binding events if peaks were random with respect to gene elements. (C) Functional clustering of Rib-bound SG genes by GO term from the DAVID analysis. The top 10 GO terms with their associated enrichment scores are shown. The top category represents binding of Rib to RPGs with a nearly sevenfold greater enrichment than the next-ranked category. See Table S1 for metadata. (D) Rib binds to the TSS of RPGs. Open circles indicate the distance between the Rib binding peak and the TSS (+1 bp) for individual genes. Red line indicates the median distance from the peak to TSS. (E) Enrichment of TSS-proximal Rib binding across the top 10 functional classes. Including the ribosome, 7 of the top 10 enriched GO classes primarily show Rib binding to the TSS. Open circles indicate the distance between the Rib binding peak and the TSS for individual genes. Red lines indicate the median distance from the peak to TSS. n = number of peaks versus (number of genes).