Purification of GFP-RNH1. GFP-RNH1 was purified by Ni–nitrilotriacetic acid (Ni-NTA) superflow (left), followed by SP sepharose fast flow (right), and 5 μl of the indicated fractions were resolved on a 10% Tris-glycine SDS-PAGE gel and stained with Coomassie G-250 stain. Purification fractions analyzed included L, column load; FT, flow through; 1–7 or 1–12, eluted fractions; and B, leftover beads. The GFP-RNH1 protein is indicated by arrows. Mw, molecular weight in kDa.