Lack of effect of chronic treatment with GSM compound 2 in PSAPP mice on APP (FL)/tubulin, α-CTF/tubulin, and β-CTF/tubulin ratios. (A–C) Quantitation of APP (FL)/tubulin ratios (A), α-CTF/tubulin ratios (B), and β-CTF/tubulin ratios (C) in 6-mo-old and 9-mo-old PSAPP mouse brain extracts following chronic treatment with either vehicle (V) or compound 2 (Cmpd 2) via Bio-Rad ImageLab software. Statistical analysis was performed using GraphPad Prism software, and the results are expressed as the mean of the ratio (black horizontal line) ± SEM. (D) Immunoblots of PSAPP brain extracts from 6-mo-old or 9-mo-old PSAPP mice treated with either vehicle (V) or compound 2 (2). Blots were probed for APP (FL), α-CTFs, β-CTFs, and tubulin using anti-carboxyl terminal APP and anti-tubulin antibodies.