Sera from APS-1 patients do not influence IFN-β_1, IFN-λ_1, IFN-λ₂, and IFN-λ₃ antiviral activity but block IFN-α and IFN-ω antiviral activity. The effect of APS-1 serum (white bars) was evaluated in a reconstituted HAE model of SARS-CoV-2 infection in comparison with a control condition (black bars). (A) Nasal HAEs were treated (24 h before and 1 h after SARS-CoV-2 infection) with recombinant IFN-α₂, IFN-β₁, IFN-λ₁, IFN-λ₂, IFN-λ₃, or IFN-ω in the presence or absence of APS-1 serum. Apical washes were performed at 54 hpi, and viral titers were determined by RT-PCR. Results are representative of three biological replicates and expressed as relative to the mock-treated control. (B) Apical infectious viral titers at 54 hpi determined by TCID50. The dotted line depicts the limit of detection. (C) Δ TEER between t = 0 and t = 54 hpi. (D) Relative expression ofIFI44L assessed using FilmArray technology from total cellular RNA extracted after infection. In the figure, bars and error bars represent mean and SD, respectively. Multiple t tests with Bonferroni–Dunn method were used for the statistical analysis of data presented in this figure (comparison between control and APS-1 serum conditions in each readout; **, P < 0.01; ***, P < 0.001).