Deletion of Coro1B and Coro1C do not directly affect NMIIA localization and activity. (A) Still frames of parental and null cells (top) plated on 8 kPa polyacrylamide gels. Corresponding still images of fluorescent beads below. (B) Western blot analysis of myosin light chain (MLC) and phosphorylated MLC (pMLC) in parental and null populations. pMLC/MLC ratios are provided below. (C) Bar graph representing quantification of pMLC to total MLC ratios across five independent experiments. (D and E) Integrated fluorescence intensity of (D) MLC and (E) pMLC in parental (n = 181) and null (n = 193) cells. (F) Representative immunofluorescent staining of non-muscle myosin IIA, vinculin and F-actin in parental and null cells; Scale bar, 10 μm. Yellow arrowheads mark accumulation of NMIIA along actin bundles associated with focal adhesions. For all beeswarm superplots, the mean of experimental replicates are color-coded and overlayed on violin plots representing cumulative cell level data. For all graphs, Student’s t tests were performed, and error bars denote SEM, unless otherwise stated. Source data are available for this figure: SourceData FS3.