Loss of Coro1B/1C increases contractility. (A) Representative collagen contractility gels. (B) Quantification of collagen gel area, 24 h post-plating, from control (n = 10), parental (n = 24), and null (n = 20) population, acquired from three independent experiments. One-way ANOVA with Dunn’s multiple comparison test performed. Error bars show 95% C.I. (C) Representative traction force maps of parental and null cells plated on 8 kPa polyacrylamide hydrogels. Scale bar, 50 μm. Scale (right) shows traction force magnitude in pascals. (D) Average strain energy density (in femtojoules per micron squared) of parental (n = 40) and null (n = 24) cells extracted from traction force map in C. Data represents two independent experiments with two technical replicates in each experiment. (E) Immunofluorescent staining of vinculin and F-actin in parental and null populations. Scale bar, 100 μm. Lower panels are 2× magnifications of vinculin and F-actin from boxed regions. (F) Graph depicts number of vinculin-positive adhesions per cell; parental (n = 19) and null (n = 18) cells. (G–I) Quantification of (G) vinculin fluorescence intensity; (H) area (in micron squared); and (I) aspect ratio of parental (n = 49) and null (n = 50) cells. For all beeswarm superplots, the mean of experimental replicates are color-coded and overlayed on violin plots representing cumulative cell-level data. Error bars denote SEM. For all graphs, Student’s t tests were performed. **P value <0.005, ***P value <0.0005, ****P value <0.0001.