Deletion of Coro1B and Coro1C do not significantly impact Arpc2 and cofilin levels in fibroblasts. (A) Western blot analysis of Arpc2 in parental and null cells. (B) Width of Arpc2 at the edge in microns of parental (n = 22) and null (n = 24) cells. (C) Blot analysis of total cofilin and phospho-cofilin (p-cofilin) in parental and null cells. Average ratio of p-cofilin to total cofilin levels are depicted below blot. (D) Quantification of p-cofilin/cofilin ratios from parental and null populations. Each dot represents an independent experiment and error bars denote SD. (E) Quantification of the ratio between cofilin and F-actin fluorescence at the leading edge of parental (n = 46) and null (n = 46) cells. (F) Fluorescence intensity profiles of cofilin and actin in parental (n = 7) and null (n = 8) cells within 3 μm from the cell edge. Black dotted lines denote the average peak in F-actin intensity and black arrows depict a similar peak in cofilin fluorescence in parental and null cells. (G) Quantification of polymerization rates in microns per minute of parental and null cells in the presence and absence of 100 nM jasplakinolide; n > 25. Individual cell data from three experimental replicates are overlayed on bar graph, and error bars denote SD. Two-way ANOVA was performed. For all beeswarm superplots, the mean of experimental replicates are color-coded and overlayed on violin plots representing cumulative cell level data. For all graphs, Student’s t tests were performed, and error bars denote SEM, unless otherwise stated. *P value <0.04, ****P value <0.0001. Source data are available for this figure: SourceData FS2.