Loss of Coro1B/1C affects random and directed migration and lamellipodia dynamics. (A) Sample plot depicting the tracks of parentals (cyan) and null (magenta) cells migrating on 10 μg/ml FN. (B) Beeswarm superplot depicting random migration velocity of parental (n = 223) and null (n = 300) fibroblasts. The mean of each biological replicate is color-coded and overlayed on violin plots. Error bars represent the standard error of mean. (C) Rose plots for parental (n = 102) and null (n = 139) cells on a FN gradient. (D) FMI graph (mean ± 95% confidence interval) for haptotaxis of cells on FN gradient. *P value = 0.04. (E) Example kymographs for parental and null cells. White and black arrows represent protrusion and retraction, respectively. Scale bar, 5 μm. (F–H) Beeswarm superplots of lamellipodial dynamics in parental (n = 17) and null (n = 16) cells showing (F) protrusion rate in microns per minute, (G) retraction rate in micron per minute and, (H) protrusion duration in minutes. For all graphs, Student’s t tests were performed. Error bars represent the SEM. ****P < 0.0001.