F-actin regulators Coro1B and Coro1C are regulators of cell motility in fibroblasts. (A and B) Relative expression of (A) human Coro1B-GFP and (B) endogenous Coro1C in parental and null populations normalized to GAPDH loading control. Plotted as mean ± SEM. (C) Cell growth represented as number of cells (×10⁵) in parental and null populations over time. Three independent experiments were performed in duplicates. (D) Cell spread area (in microns squared) of parental and null cells over 3 h after plating on 10 μg/ml FN. Two independent experiments were performed; n > 300 cells per time point. C and D are plotted as mean with 95% CI. (E) Total distance traveled by parental (n = 223) and null (n = 300) cells in microns. (F) Schematic of mixed parental and null cell populations in the microfluidic haptotaxis chamber. (G) Velocity in microns per hour (left) and total distance traveled in microns (right) of parental (n = 102) and null (n = 139) cells from haptotaxis assays. (H) Protrusion distance of parental (n = 17) and null (n = 16) cells plated on 10 μg/ml uniform FN. For beeswarm superplots, means of experimental replicates are color-coded and overlayed on violin plots respresenting cumulative cell level data. Error bars denote SEM. Student’s t tests were performed for graphs A and B and E–H. *P value = 0.028; **P value = 0.0013; ***P value = 0.0007; ****P value <0.0001.