Generation and characterization of Coro1B and Coro1C matched pair cell lines. (A) Schematic representation of 4-hydroxytamoxifen-induced deletion of Coro1B-GFP and Coro1C. (B) Western blot analysis of matched-pair MTFs with and without Coro1B-GFP and Coro1C. (C) Confocal micrographs showing mixed populations of Parental and Null cells immunostained for Coro1C and phalloidin (F-actin visualization). Only parental cells express Coro1B–GFP and Coro1C. White asterisks denote null cells. Scale bar, 25 μm. (D and E) Representative images for Coro1B-GFP, Coro1C, Arpc2, and F-actin in parental cells. (D) Insets show Coro1B-GFP and Coro1C in magnified regions of the lamellipodia. Scale bar, 5 μm. (E) Parental cells after 30-min treatment with 150 μM CK666 or DMSO. Yellow arrowheads denote the lack of Coro1B or Coro1C along linear actin cables. Scale bar, 30 μm. Source data are available for this figure: SourceData F1.
Figure 1.

Generation and characterization of Coro1B and Coro1C matched pair cell lines. (A) Schematic representation of 4-hydroxytamoxifen-induced deletion of Coro1B-GFP and Coro1C. (B) Western blot analysis of matched-pair MTFs with and without Coro1B-GFP and Coro1C. (C) Confocal micrographs showing mixed populations of Parental and Null cells immunostained for Coro1C and phalloidin (F-actin visualization). Only parental cells express Coro1B–GFP and Coro1C. White asterisks denote null cells. Scale bar, 25 μm. (D and E) Representative images for Coro1B-GFP, Coro1C, Arpc2, and F-actin in parental cells. (D) Insets show Coro1B-GFP and Coro1C in magnified regions of the lamellipodia. Scale bar, 5 μm. (E) Parental cells after 30-min treatment with 150 μM CK666 or DMSO. Yellow arrowheads denote the lack of Coro1B or Coro1C along linear actin cables. Scale bar, 30 μm. Source data are available for this figure: SourceData F1.

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