Effects of expression of MLC2 mutants in wild-type DLD1 cells. (A) Stable lines of wild-type DLD1 cells transfected with the indicated GFO-tagged MLC2 mutants were immunostained for GFP and ZO-1. (B) Migration distance of these cells at the wound edges 24 h after scratching of the culture. Data were analyzed as explained in the legend of Fig. 1 B. Error bars represent SEM. Scale bars, 10 µm.