Effect of E-cadherin deletion on other proteins. (A) Effect of E-cadherin deletion on p120-catenin level. Wild-type and E-cad KO Caco2 cells were mixed and coimmunostained for E-cadherin (E-cad) and p120-catenin, which allows direct comparison of the level of these proteins between the two cell populations. Graph, relative immunostaining intensity of p120-catenin. A few points in each of the bicellular junctions were randomly selected for measurement, using six wild-type and seven E-cad KO cells. ****, P < 0.0001 (t test, one-sided). (B) Coimmunostaining for ZO-1 and claudin 7 or 34/ARPC2 and actin in wild-type and E-cad KO Caco2 cells. Scale bars, 10 µm.