Distribution of merlin, Rac1, and YFP-PBD.(A and B) Coimmunostaining for merlin and actin in a layer of wild-type (A) or E-cad KO (B) Caco2 cells that is engaging in wound healing. The squared portion is enlarged at the right in each of the figure sets. Brackets indicate junctional sites where protrusions emerge. (C) Coimmunostaining for Rac1 and actin. Rac1 immunostaining signals disappeared when cells were pretreated with Rac1 siRNA, verifying the significance of the data. (D) Covisualization of Abi1 and YFP-PBD transiently introduced into the cells. White and yellow brackets indicate examples of the junctional sites where Abi1-positive protrusions are detected and undetected, respectively. Arrows point to representative sites where Abi1 and YFP-PBD overlap. Nuclear stains in YFP-PBD–transfected cells are not identified. Scale bars, 10 µm.
Figure S5.

Distribution of merlin, Rac1, and YFP-PBD. (A and B) Coimmunostaining for merlin and actin in a layer of wild-type (A) or E-cad KO (B) Caco2 cells that is engaging in wound healing. The squared portion is enlarged at the right in each of the figure sets. Brackets indicate junctional sites where protrusions emerge. (C) Coimmunostaining for Rac1 and actin. Rac1 immunostaining signals disappeared when cells were pretreated with Rac1 siRNA, verifying the significance of the data. (D) Covisualization of Abi1 and YFP-PBD transiently introduced into the cells. White and yellow brackets indicate examples of the junctional sites where Abi1-positive protrusions are detected and undetected, respectively. Arrows point to representative sites where Abi1 and YFP-PBD overlap. Nuclear stains in YFP-PBD–transfected cells are not identified. Scale bars, 10 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal