Expression of WRC and Arp2/3 complex, effect of Rac1 inhibitor, and effect of NAP1 or p34/ARPC2 depletion. (A) Western blots for WAVE2, Nap1, or p34/ARPC2 (p34) in wild-type, αEcat KO, and E-cad KO Caco2 cells. The Nap1 blot was obtained separately from the WAVE2 and α-tubulin blots. (B) Western blots for p34/ARPC2 in p34/ARPC2 siRNA-treated cells. (C) Effect of the Rac1 inhibitor EHT1864 on actin, Abi1, and p34/ARPC2 distribution in wild-type or αE-cat KO Caco2 cells. Cells were incubated with 100 µM EHT1864 for 5 h 30 min. (D) Effect of Nap1 or p34/ARPC2 depletion on the level of other proteins. Scale bars, 10 µm.