Centrobin removal suppresses ectopic spindle pole formation in TRIM37Δ cells. (A) Images of mitotic cells labeled for microtubules, centrobin, and DNA for the indicated conditions. Scale bar, 5 µm. (B) Quantification of ectopic spindle pole frequency for the indicated conditions. The experiment was performed once. (C) Stills from live imaging of mitotic chromosome dynamics for the indicated conditions; time 0 is nuclear envelope breakdown (NEBD). Transient multipolarity is evident in the chromosome configuration (arrow) in a subset of TRIM37Δ cells. Scale bar, 10 µm. (D) Quantification of transient multipolarity and multipolar segregation (left graph) and of mitotic duration (right graph), defined as the time from NEBD to chromosome decondensation. Mean and 95% CI for mitotic duration are plotted on top of the values for individual cells; P values are from unpaired t tests. The experiment shown in C was performed twice with similar outcomes; data from one experiment are quantified in D. ****, P < 0.0001.