Cryo-EM structures of 7-155 and 7-176 in complex with BG505 SOSIP.664 trimer. (A) Side view (left) and top view (right) of the 6.7-Å single-particle cryo-EM reconstruction of HIV-1 BG505 SOSIP.664 Env in complex with 7-155 IgG Fab. (B) Same as in A but for the 7.0-Å cryo-EM reconstruction of Env-7-176-IgG Fab complex. (C) Model of the BG505 SOSIP.664-7-155 complex (side view) obtained by fitting the BG505 SOSIP.664 structure and homology models from the antibodies’ variable regions and the BG505 SOSIP.664 structure into their respective single-particle reconstructions. The glycosylated BG505 SOSIP.664 structure was taken from the complex with 7-269, and some mannose residues were trimmed to avoid clashes with the modeled antibody. (D) Same as in C but for BG505 SOSIP.664-7-176 complex. (E) Superposition of the reconstructions obtained for the three BG505 SOSIP.664-bNAb complexes studied (side and top views), showing the overlap between their respective binding sites. For a better comparison, the data from the BG505 SOSIP.664-7-269-3BNC117 complex was reprocessed with a resolution limit of 7 Å.