HIV-1 neutralization by pt7 serum and monoclonal antibodies. (A) Table shows the neutralizing activity of serum IgGs and IgAs purified from six viremic controllers against selected clade B viruses (n = 5). IC₅₀ values (µg/ml) are indicated in cell center. Darker colors indicate higher potency; white, no neutralization. (B) Tables comparing ELISA binding to HIV-1 Env proteins (top) and neutralizing activity against selected clade B viruses (n = 5, bottom) of monoclonal antibodies cloned from pt7 blood IgG⁺ (red) and IgA⁺ (blue) memory B cells. 7-125^S and 7-216^S, SOSIP-isolated antibodies. Darker colors indicate higher Env reactivity or neutralization potency; white, no binding or neutralization. ELISA AUC and IC₅₀ values (µg/ml) are indicated in cell center. Dotted links indicate clonally related members. 10-1074 is the positive control. (C) Table shows the neutralizing activity of selected antibodies produced as recombinant IgGs against 12-virus reference panels. IC₅₀ and IC₈₀ values (µg/ml) are indicated in cell center. Darker colors indicate higher potency; white, no neutralization. (D) Same as C but for clade B viruses only and 7-319 IgG, 7-269 IgG and IgA antibodies. IC₅₀ values (µg/ml) are shown. (E) Same as B but for 7-107 clonal variants expressed as IgGs. (F) Same as C but for 7-269 IgA against a cross-clade extended panel (n = 56). For each viral strain, amino acids at PNGS N295 and N332 are indicated. (G) Same as F but with clade B viruses from three epidemic periods.