CEP90 forms a complex with OFD1 and MNR. (a) Schematic depicting workflow used to identify CEP90 interactors at centriolar satellites and centrioles using WT and PCM1^−/− RPE1 cells. PCM1^−/− cells localize CEP90 to the distal centriole, and WT cells localize CEP90 to the distal centriole and centriolar satellites. (b) Venn diagram comparing high-confidence interactors of CEP90 in WT and PCM1^−/− RPE1 cells. (c) Interactome dot representation of selected CEP90 interactors identified in the proteomic screen. Hits were grouped based on their cellular localization. Average number of peptide spectra (AvgSpec) is represented by dot shade. Abundance of a peptide spectrum produced in relation to the most abundant spectrum is depicted by dot size. Bayesian false discovery rate (BFDR) is represented by rim color. (d) Immunoblot of a subset of CEP90 interactions identified by proteomics were validated by coimmunoprecipitation (IP). CEP90 interacts with PCM1, OFD1 and MNR, but not α-tubulin or GAPDH. FT, flow through. Specific MNR band is indicated with an asterisk. The top band is nonspecific, as it is undiminished in the MNR-knockout cell lysates. (e) Coulson plot showing the phylogenetic distribution of a subset of centriolar proteins in select ciliated metazoan species. Orthologues identified with high confidence are indicated with a filled circle, and a subset of CEP90 interactors further explored in this study are highlighted in blue. The dendrogram on top (made using interactive tree of life; Ciccarelli et al., 2006) shows the evolutionary relationship between species.