Metabolic parameters and insulin sensitivity of PLD2-inhibited or adipocyte PLD2-ablated mice. (A–C) The levels of plasma AST and ALT (A), RER (B), and locomotor activity (C) of vehicle- or CAY10594-injected obese mice (n = 6/group). (D and E) Blood glucose concentrations during O-GTT (D) and ITT (E) based on lean mass in fasted vehicle- or CAY10594-injected obese mice (n = 6/group). (F) Inflammation-related gene expression in epiWAT from vehicle and CAY10594-injected obese mice (n = 6/group). (G–I) The level of EE (G), RER (H), and locomotor activity (I) of HFD-fed (12 wk) control and Pld2 Ad-KO mice (n = 6/group). (J and K) Blood glucose concentrations during O-GTT (J) and ITT (K) based on lean mass in HFD-fed (12 wk) control and Pld2 Ad-KO mice (n = 6/group). (L) Inflammation-related gene expression in epiWAT from HFD-fed (12 wk) control and Pld2 Ad-KO mice (n = 6/group). The data are presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by two-tailed Student’s t test (A–C, F–I, and L) and two-way ANOVA (D, E, J, and K). Data are representative of two (A, F, and L) or three independent experiments (D, E, J, and K). Veh, vehicle.