IRF8 is required for Mycl induction and maintenance by pDCs but not cDC2s. (A–D) Flow cytometry analysis BM populations from mice of the indicated Mycl-GFP and Irf8 genotypes. (C and D) Quantification of frequency of MYCL-GFP⁺ cells gated in A and B, respectively. (E–G) Flow cytometry analysis of MYCL-GFP expression by pDCs (SiglecH⁺Ly6D⁺), cDC1s (CD24⁺CD172a⁻), and cDC2s (CD172a⁻) from the spleens of mice with the indicated genotypes. (G) MFI of MYCL-GFP expression for representative populations from E and F for the indicated genotypes. Data are representative of two independent experiments (n = 3–6).