Mycl-GFP positive progenitors exist across heterogeneous populations of pDC and cDC1 restricted progenitors. (A) GFP expression by bulk Lin⁻CD135⁺ BM populations in Mycl^+/+ and Mycl^GFP/+ mice. (B) Percentage of bulk BM pDCs among Mycl-GFP population gated in A. (C) Definition of monocytes (Mono) and cMoPs analyzed in D. (D)Mycl-GFP expression by the indicated populations. (E) Frequency of Mycl-GFP⁻ CDPs (GFP⁻CD115⁺) and Mycl-GFP⁺ progenitors (GFP⁺) among Lin⁻CD11c⁻MHCII⁻CD135⁺CD117^int BM cells (n = 10). (F) Frequency of CD127⁺ CLPs and CD115⁺ CDPs among Mycl-GFP⁺ progenitors shown in Fig. 1 A. (G) Frequency of pre-pDCs (Ly6D⁺SiglecH⁺) among CD117^int and CD117^low CLPs. All data are representative of at least three independent experiments (n = 5–10).