Loss of LUBAC does not affect centrosome structure and TZ assembly (related to Fig. 2 ). (A–H) RPE-1 cells were transfected with control or LUBAC component siRNAs and serum starved for 48 h. The cells were stained with the indicated antibodies. Scale bars, 1 µm. (A) The localization of pericentrin was normal in LUBAC-depleted RPE-1 cells. (B and C) The localization of subdistal appendage proteins ODF2 and FBF1 were not affected by LUBAC depletion. (D) The localization of IFT20 on the centrosome appeared normal in LUBAC-depleted cells. (E) LUBAC is not essential for the recruitment of TTBK2 to the mother centriole. (F–H) Depletion of LUBAC could not affect the localization of TZ proteins MKS1, TMEM67, and NPHP8. Data are presented as mean ± SD of three independent experiments. Significance tested by one-way ANOVA with Dunnett’s multiple comparisons. Ctrl, control; tub, tubulin.