Binding of Ser352 mutants of GAS2L1 to EB1. Bio-GFP-tagged GAS2L1 constructs were transiently expressed in HEK293T cells and pulled down using streptavidin beads. Aliquots of the pull-downs (50%) and lysate inputs (2%) were immunoblotted for GFP and EB1. WT, GAS2L1 WT.