Analysis of purified bacterially expressed GAS2L1 fragments. (A) GAS2L1 fragments harboring a His₆-FLAG or His₆ tag were expressed in bacteria and purified through the His₆ tag. Purified proteins were analyzed using SDS-PAGE and Coomassie Blue staining. Arrowheads: recombinant proteins. (B) Recombinant GAS2L1 fragments (His₆-tagged) were used in F-actin or microtubule sedimentation assays in the absence of F-actin or microtubules, respectively, in actin polymerization buffer (F-actin buffer) or BRB80 buffer (microtubule buffer). Pellets and supernatants were collected after sedimentation for anti-His₆ immunoblotting.