Ser-to-Ala substitution at position 355, 357, 358, or 360 does not affect centrosome-separating activity of GAS2L1. RPE-1 cells were transfected with GFP-tagged GAS2L1 WT or mutants (S355A, S357A, S358A, and S360A), and stained with an anti-γ-tubulin antibody. Centrosome distance was measured from three independent experiments: n = 110 (WT), 114 (S355A), 121 (S357A), 98 (S358A), and 104 (S360A).