Additional characterization of NPM1 lysate granules and RNA composition. (A) Western blot of U2OS lysate (supernatant) and pellet run against a purified NPM1 standard. Concentrations were assessed by densitometry to be ∼8.4 µM NPM1 in the cell (7.7 µM in soluble fraction and 0.7 µM in the pellet). (B) Addition of purified NPM1 protein (increasing concentration by 15 µM) to a cell lysate stably expressing GFP induces LLPS, but GFP does not localize to NPM1-induced lysate granules as visualized with DIC. Scale bar, 10 µm. (C) RNA composition of stress granules (Khong et al., 2017; left), G3BP1 lysate granules (center), and NPM1 lysate granules (right) analyzed by RNA-seq.