Gish knockdown increased Rho-GFP level. (A–B”) Control wing discs (hh-Gal4; A–A”) or wing discs expressing UAS-Gish-RNAi under the control of hh-Gal4 (B–B”) were immunostained for Ci (red) and Rho-GFP (green). hh-Gal4 drives UAS transgene expression in P-compartment cells marked by the lack of Ci expression.