AIM2 inhibits type A EAE development. (A) Mean clinical scores of age-matched female WT, AIM2^−/−, and ASC^−/− mice subjected to type A EAE (n = 8 mice per group). (B) Representative H&E and LFB staining and histology scores of spinal cord sections from type A EAE–induced WT, AIM2^−/−, and ASC^−/− mice, showing inflammatory cell infiltration and demyelination (arrowheads). Scale bar, 500 µm. (C and D) Flow-cytometric analysis of immune cells (including CD45⁺CD4⁺ T cells, CD45⁺CD8⁺ T cells, and CD45⁺CD11b⁺ microglia and myeloid cells) infiltrated to the spinal cord and brain of type A EAE–induced WT, AIM2^−/−, and ASC^−/− mice at day 18 after immunization (n = 6 mice per group). Data are presented as representative plots (C) and summary graphs of quantified percentages and absolute cell numbers (D). (E) ELISA analysis of IL-1β production in serum from indicated mice in type A EAE (n = 5 mice per group). Data are pooled from three independent experiments. **, P < 0.01; ***, P < 0.001. Error bars show mean ± SEM. Unpaired t test for A and E, and multiple unpaired t test for D.