Colocalization of MOSPD2 with different organelles. (A–D) Colocalization in HeLa cells transfected with GFP-MOSPD2 (green) of MOSPD2 and endogenous EEA1 (A, magenta), Lamp1 (B, magenta), OPA-1 (C, magenta), and GM130 (D, magenta). Subpanels on the right are higher magnification images of the outlined areas. The overlay panel shows merged channels. The coloc panel displays a colocalization mask in which pixels of the green and magenta channels that co-localize are shown in white. Linescan shows fluorescence intensities of the green and magenta channels along the white arrow from the overlay subpanel. Black rectangles indicate the position of early endosomes (EE; A), late endosomes (LE; B), mitochondria (Mito.; C), and the Golgi apparatus (Golgi; D). Scale bars: 10 µm (insets, 2 µm). Images were acquired on a confocal microscope (Leica SP5, 63× NA 1.4).
Figure S2.

Colocalization of MOSPD2 with different organelles. (A–D) Colocalization in HeLa cells transfected with GFP-MOSPD2 (green) of MOSPD2 and endogenous EEA1 (A, magenta), Lamp1 (B, magenta), OPA-1 (C, magenta), and GM130 (D, magenta). Subpanels on the right are higher magnification images of the outlined areas. The overlay panel shows merged channels. The coloc panel displays a colocalization mask in which pixels of the green and magenta channels that co-localize are shown in white. Linescan shows fluorescence intensities of the green and magenta channels along the white arrow from the overlay subpanel. Black rectangles indicate the position of early endosomes (EE; A), late endosomes (LE; B), mitochondria (Mito.; C), and the Golgi apparatus (Golgi; D). Scale bars: 10 µm (insets, 2 µm). Images were acquired on a confocal microscope (Leica SP5, 63× NA 1.4).

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