MOSPD2 is enriched around LDs in different cell lines. (A) HeLa cells expressing GFP-MOSPD2 (green) and not treated with OA were labeled with Nile Red to stain LDs (magenta). (B–D) Localization of GFP-MOSPD2 (green) in Huh-7 (B), 501-MEL (C), and MCF7 (D) cells. Cells were either treated with OA (right) or not treated (left). LDs were stained using Nile Red (magenta). Subpanels on the right are higher magnification images of the outlined areas. The overlay panel shows merged channels. The coloc panel displays a colocalization mask in which pixels of the green and magenta channels that co-localize are shown in white. Linescan shows fluorescence intensities of the green and magenta channels along the white arrow from the overlay subpanel. Black rectangles indicate the position of LDs. (A–D) Scale bars: 10 µm (insets, 2 µm). Confocal microscope (Leica SP5, 63× NA 1.4) images.
Figure S1.

MOSPD2 is enriched around LDs in different cell lines. (A) HeLa cells expressing GFP-MOSPD2 (green) and not treated with OA were labeled with Nile Red to stain LDs (magenta). (B–D) Localization of GFP-MOSPD2 (green) in Huh-7 (B), 501-MEL (C), and MCF7 (D) cells. Cells were either treated with OA (right) or not treated (left). LDs were stained using Nile Red (magenta). Subpanels on the right are higher magnification images of the outlined areas. The overlay panel shows merged channels. The coloc panel displays a colocalization mask in which pixels of the green and magenta channels that co-localize are shown in white. Linescan shows fluorescence intensities of the green and magenta channels along the white arrow from the overlay subpanel. Black rectangles indicate the position of LDs. (A–D) Scale bars: 10 µm (insets, 2 µm). Confocal microscope (Leica SP5, 63× NA 1.4) images.

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