Images and plots of side-averaged Golgi proteins. HeLa cells expressing indicated exogenous proteins were nocodazole-treated and immunostained for giantin and the indicated proteins. (A–D) Side and en face averaged ERES and ERGIC (LQ < −0.25; A), cis-Golgi (−0.25 ≤ LQ <0.25; B), medial-Golgi (0.25 ≤ LQ <0.75; C), and trans-Golgi proteins (0.75 ≤ LQ <1.25; D). In each panel, the first row shows side-average images. White, blue, and red horizontal lines represent fluorescence centers of the protein of interest, GM130, and GalT-mCherry, respectively. The second row shows the axial line intensity profile (Axial). The origin corresponds to the fluorescence center of the protein of interest. Intensity is normalized, and the unit of the axial distance is pixels. The third row shows the lateral line intensity profile (Lateral). Intensity is normalized, and the origin is at the Golgi axis. The x coordinate was normalized by half of the lateral size of giantin. The fourth row shows en face average images. The fifth row displays the radial mean intensity profile (Radial). The distance from the center was normalized by the radius of giantin. The number of ministacks analyzed, n, is indicated in the upper right corner of each average image. (E and F) Merged side-average images. Blue and red horizontal lines represent the fluorescence centers of GM130 and GalT-mCherry, respectively. (G) Side and en face averaged TGN proteins (LQ ≥ 1.25). Panels are organized as A–D. Proteins are arranged by their LQs. Scale bar, 200 nm.