H₂O₂-induced INAVA condensates colocalize with conjugated ubiquitin: control experiments for βTrCP2 colocalization. (A) HCT8-INAVA-GFP cells were treated with H₂O₂ (1 mM) for 90 min and stained with conjugated ubiquitin antibody FK2. Scale bar = 10 μm. (B) Control images for Fig. 4 B of cells expressing only INAVA-GFP. HCT8-INAVA-GFP cells expressing doxycycline (Dox)-inducible myc-βTrCP2 without Dox treatment. Cells treated with condensate inducers as in Fig. 4 B. Cells were fixed and stained with phalloidin-TRITC (F-actin) and α-myc. (C) HCT8 cells stably expressing Dox-inducible myc-βTrCP2. Cells were treated overnight with Dox (1 μg/ml), fixed, and stained with phalloidin-TRITC and α-myc. Scale bar = 10 μm.