Gene expression and profiles of T cell subsets. (A) tSNE plot of 64,449 tumor and peripheral-derived T cells derived from four mice, colored as 33 distinct phenotypic clusters. (B) Distribution of the percentage of cells in each cluster from individual animals. (C) tSNE plot of key marker gene expression. (D and E) Violin plots of major population and cluster-specific marker genes in CD8⁺ (D) and CD4⁺/T reg cell clusters (E).