BFAR deficiency does not affect Th19 and Treg cell differentiation. (A and B) Flow cytometric analysis of the inducibility of Th1, Th2, Th17, or Treg cells of WT and BFAR-deficient naive CD4⁺ T cells under different differentiation conditions for 3 d. (C and D) Flow cytometric analysis of Th17 and Treg cell inducibility of mouse naive CD4⁺ T cells that were left pretreated with 0.5 ng ml⁻¹ TGFβ1 (Lo) or 10 ng ml⁻¹ TGFβ1 (Hi) for 12 h and then cultured under Th17 and Treg cell conditions for 3 d. (E–G) Flow cytometric analysis of the inducibility of Th17 and Treg cells of WT and BFAR-deficient naive CD4⁺ T cells under different differentiation conditions for 3 d with the indicated doses of TGFβ1. (H) ELISA measurements of supernatant TGFβ1 and IL-10 levels of Treg cells of WT and BFAR-deficient naive CD4⁺ T cells under Treg cell differentiation conditions for 3 d with the indicated doses of TGFβ1. Each panel is representative of two independent experiments. Student’s t test was used. Bars, mean; error bars, SEM; ns, not significant.