NE ultrastructure at sites of Atg39 accumulation (supplemental to Fig. 9 ). (A) Bar plot of the quantification of corrected total cell fluorescence of endogenous Atg39-GFP along with overexpressed Atg39-GFP (high expression) and GFP-Atg39 (low expression). Corrected total cell fluorescence from 75 cells plotted. (B–F) CLEM tomograms from cells expressing low or high levels of Atg39 as indicated. i: Fluorescence micrograph with arrowheads pointing to ROIs similarly annotated in corresponding EM tomogram. ii: Virtual slice from electron tomogram with the location of fluorescence from i indicated by similarly colored arrowheads. iii: Magnification of boxed view in ii. iv: Annotation of virtual slices from iii, with the ONM in blue, the INM in teal, vacuole in green, and mitochondria in orange. Arrow points to continuity of ONM. Asterisks denote nuclear pores. Scale bars are 100 nm.