Figure 4.
Endogenous Atg39 accumulates in NE blebs independently of Atg11.(A) Deconvolved fluorescence micrographs of Atg39-GFP in the indicated strains cultured in medium lacking nitrogen (SD-N) for 4, 8, and 24 h. Scale bars are 5 μm. Arrows point to NE blebs. (B) Bar chart plotting the percentage of cells with NE blebs in the indicated strains from experiments in A. At least 50 cells of each genotype were quantified from three independent experiments. Mean and error bars denoting SD are shown. (C) Scatter plot of the quantification of Atg39-GFP fluorescence intensity in NE blebs in the indicated strains from experiments in A. At least 50 cells of each genotype were quantified from three independent experiments. Mean and error bars denoting SD are shown. **, P ≤ 0.01; ****, P ≤ 0.0001 by one-way ANOVA.

Endogenous Atg39 accumulates in NE blebs independently of Atg11. (A) Deconvolved fluorescence micrographs of Atg39-GFP in the indicated strains cultured in medium lacking nitrogen (SD-N) for 4, 8, and 24 h. Scale bars are 5 μm. Arrows point to NE blebs. (B) Bar chart plotting the percentage of cells with NE blebs in the indicated strains from experiments in A. At least 50 cells of each genotype were quantified from three independent experiments. Mean and error bars denoting SD are shown. (C) Scatter plot of the quantification of Atg39-GFP fluorescence intensity in NE blebs in the indicated strains from experiments in A. At least 50 cells of each genotype were quantified from three independent experiments. Mean and error bars denoting SD are shown. **, P ≤ 0.01; ****, P ≤ 0.0001 by one-way ANOVA.

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