NMY-2 accumulates adjacent to lobe necks as they begin to ingress. (a–a’’) Live image of Mem^PGC and NMY-2-YFP^PGC at the onset of lobe formation. NMY-2-YFP^PGC can be seen accumulating at the membrane adjacent to where lobe necks are beginning to ingress (arrows). Scale bar, 5 µm.