Tum recruitment to wounds depends on actin. (A–D”) Localization of sfGFP-Tum and an actin reporter (sChMCA) upon injecting buffer control (A–A”), colchicine (B–B”), LatB (C–C”), or colchicine + LatB (D–D”) in NC4–6 staged embryos. Time after wounding is indicated. Scale bar: 20 µm. (E) Coomassie-stained SDS-PAGE gels of proteins used in this study.