Quality control and characterization of cells in scRNA-seq. (A) Statistics of cells analyzed in scRNA-seq. (B) Violin plots for internal control genes used the comparison of cells from control and Vegfr2^iΔEC mice. (C) Pearson correlation matrix of the average expression profiles of all subpopulations based on all differentially expressed genes. (D) UMAP plots showing marker genes. (E) qPCR analysis of freshly isolated dental pulp cells at P12 (control [Cont] n = 7, Vegfr2^iΔECn = 7), confirming the scRNA-seq results. (F) UMAP plots showing expression of Vegfa and Noggin in reclustered EC1 and EC2 cells in Fig. 7 B. Data are presented as means ± SD. Comparisons between two groups were evaluated using a two-sided Student’s t test.