Par-1 is required for both Dia and actin to form cap bundles, whereas RhoGEF2 and Rok mainly promote myosin at the borders. Dia and phalloidin staining in embryos endogenously expressing Zip-GFP. Zip-GFP recruited to actomyosin borders of control and par-1 RNAi embryos (note disorganization with par-1 RNAi). Dia and F-actin are cortical in par-1 RNAi embryos but form cap bundles of controls (arrows). Observed in 16/16 control RNAi embryos and 8/8 par-1 RNAi embryos at cycle 11. For RhoGEF2 RNAi embryos and Rok RNAi embryos, Zip-GFP was lower in borders versus control. Caps of RhoGEF2 RNAi embryos and Rok RNAi embryos displayed Dia-positive actin bundles (arrows). Note the general disorganization of the Rok RNAi caps. Observed in 16/16 RhoGEF2 RNAi embryos (combined data of two shRNA constructs) and 14/14 Rok RNAi embryos at cycle 11. Dia and phalloidin images deconvolved.