Par-1 localizes to actin cap bundles, actomyosin borders, and mitotic spindles. (A) Overexpressed GFP–Par-1 live with RNAi depletion of endogenous Par-1. Apical projections show caps (one bracketed). At interphase and prophase, GFP–Par-1 localizes to cap bundles (white arrows), a pattern that dissipates into metaphase and anaphase. Basal projections show GFP–Par-1 at the actomyosin border of each cap (yellow arrows), at centrosomes (purple arrows), and at portions of the anaphase central spindle (red arrowheads). Time = 0 is arbitrary. (B) Par-1–GFP expressed from an endogenous gene trap. At interphase, Par-1–GFP in cap bundles (white arrows and inset). At metaphase, Par-1–GFP enriched at actin cap accumulations, mitotic spindle centrosomes (purple arrows), and actomyosin borders (yellow arrow). Photobleaching of weakly expressed Par-1–GFP hindered full cell cycle imaging of the same embryo. (A and B) Images deconvolved. Time given in minutes and seconds.