RNA expression, T cell subtypes and cross-shared clones. (A) RNA-based progression scores of ITGA1 in FACS-sorted bulk T cells. Data shown as mean expression in 100 T cells per patient per time point (n = 11). (B) Representative sorting strategy of T cell subsets (T_naive = blue, T_CM = green, T_EM = orange, T_EMRA = red) for XY-FISH (Fig. 2 C), pregated on live single CD3⁺ cells, and quantification (n = 5). Data shown as percentage ± SEM of live CD3⁺ T cells. (C) Cross-shared clones of sorted T cells from pre-Tx skin and post-Tx blood at days 14, 100, and 365 after transplantation as identified by Smart-seq2/TRUST pipeline. Data shown as mean percentage ± SEM of all detected TCR clones in each sample (n = 11 patients). SSC, side scatter.