Mutant S839I mice display gut microbial dysbiosis. (A) Fecal DNA was processed to obtain 16S rRNA abundance (eight female and three male wild type mice; seven female and three male S839I mice). (B) Comparison of α diversity measured using Shannon diversity index. Statistical significance was determined using Wilcoxon rank sum test (P < 0.05). (C) Stacked-bar plot representing the phylum-level (left panel) and genus-level (right panel) relative abundance of bacterial communities in the fecal microbiome of wild type and S839I mice. (DandE) Genera that were significantly increased (D) or decreased (E) in the fecal microbiome of S839I mice. Data shown in are mean ± SD. Comparisons were done using Kruskal–Wallis test, and those with P < 0.05 are shown. (F) Predicted functional composition of the fecal microbiome. KEGG orthology function within the KEGG pathways for IL-17 signaling (ko04657), Th17 cell differentiation (ko04659), and NOD-like receptor signaling (ko04621). Data shown are mean ± SD. Statistical analysis was conducted using a Kruskal–Wallis test with Tukey-Kramer post hoc test.