Regulation of Kinesin-13 and microtubule polarity in the PLM neuron. In the posterior process of the PLM neuron, the components of Wnt signaling induce mixed polarity of microtubules through the up-regulation of KLP-7 activity, whereas in the anterior process, KLP-7 is negatively regulated by the CED-10/PAK-1 cascade in order to polarize the microtubules in a plus-end-out manner.