Microtubule polarity in the PLM neuron is reversed in wnt mutants. (A) Confocal image and illustration of neuronal polarity phenotype in lin-17(0). The red arrowhead and the white arrow are indicating the short anterior process and long posterior process, respectively, in lin-17(0) background. (B) Kymographs of EBP-2::GFP movie (Video 1) showing the microtubule growth events in lin-17(0). The green and magenta traces represent microtubules of plus-end-out and minus-end-out polarity, respectively. (C and D) The histogram shows the fraction of plus-end-out (P) and minus-end-out (M) microtubules (C) and the percentage of PLM processes with microtubules oriented in either a unipolar or mixed manner (D). N = 5–8 independent replicates; n (number of neurons) = 33–63. Ant, anterior; Pst, posterior. (E and F) Growth length and duration of microtubules measured from the EBP-2::GFP kymograph (B). N = 3 independent replicates; n (number of EBP-2 tracks) = 170–455. (G and H) Kymographs (G) and the quantification of the dynamics (H) of GFP::PTRN-1 (juEx6455) reporter in lin17(0) mutant. N = 3–5 independent replicates; n (number of neurons) = 20–27. (I) Kymographs obtained from the time-lapse movie of GFP::RAB-3 (jsIs821) reporter (Video 2) in lin-17(0) showing the events of anterograde (green arrow trace) and retrograde (magenta arrow trace) movement. White arrows show the static RAB-3 particles. The red arrowheads represent RAB-3 particles frequently switching direction. (J and K) The number of transport events of GFP::RAB-3 vesicles in anterograde (Anter) and retrograde (Retr) directions. N = 3–7 independent replicates; n (number of neurons) = 18–42. For C, E, F, H, J, and K, ***, P < 0.001; ANOVA with Tukey’s multiple comparison test. For D, ***, P < 0.001; Fisher’s exact test. Error bars represent SEM.